Mycoplasma seminis
(Fischer et al., 2020)
Etymology
Gr. n. mukes – fungus, Gr. neut. n. plasma – anything formed, N.L. neut. n. Mycoplasma – fungus form; L. gen. n. seminis – of semen
Taxonomy
Mycoplasmatales – Mycoplasmataceae – Mycoplasma – Mycoplasma seminis (Synoviae cluster), closely related to Mycoplasma verecundum (16S rRNA gene sequence similarity – 98.17%) (Fig. 1)
Type strain
ARNOT (gyrfalcon – Falco rusticolus, Germany, <2008), (Fig. 2, 16S rRNA gene sequence)
Genomes
one completed genome (2200 – Austria) (NCBI Genome deposit per 11/05/2024)
Cell morphology
spherical – coccoid
Colony morphology
fried egg morphology (Fig. 3)
Metabolism
oxidation of organic acids (pyruvate, lactate); non-fermentative, non-arginine-hydrolyzing, non-urea-hydrolyzing
Host
falcons
Habitat
semen and respiratory tract
Disease(s)
unknown, considered to be facultative pathogenic
Pathogenicity
factors unknown
Epidemiology
unknown, reported to occur rarely in semen and frequently in the respiratory tract (choanae) of falcons
Diagnosis
cultivation and species identification by MALDI-ToF MS, serology or genetically; PCR
Fig. 1. Maximum likelihood tree showing the phylogenetic position of Mycoplasma seminis ARNOT within the Synoviae cluster of Mycoplasmataceae based on 16S rRNA gene sequences. The sequence of Mycoplasma hyopneumoniae JT was used as out-group (Hyopneumoniae cluster). Numbers at nodes represent bootstrap confidence values (1000 replications). Only values > 80% are shown. Bar, number of substitutions per nucleotide position. Credits: Joachim Spergser (Vetmeduni Vienna)
TGCATGTCGAGCGGAGTTCTTCGGAACTTAGCGGCGAATGGGTGAGTAACACGTACTTAACGTACCTCTTAGATTGGGATAACGGTGAGAAATCACCGCTAATACCGGATACTTATAAAGAAGGCATCTTCTTTATATAAAAGGAGCTCTCAAGCTCCACTAGGAGATCGGGGTGCGGAACATTAGTTAGTTGGTGAGGTAATGGCTCACCAAGACTATGATGTTTAACGGGGTTGAGAGACTGATCCGTCACACTGGGACTGAGATACGGCCCAGACTCCTACGGGAGGCAGCAGTAGGGAATTTTCCACAATGGGCGAAAGCCTGATGGAGCGACACAGCGTGCAGGATGAAGGCCTTCGGGTTGTAAACTGCTGTTATTTAGGATGAAAAAATAGTAGAGGAAATGCTATTATCTTGACAGTACTAAATCAGAAAGCAACGGCTAACTATGTGCCAGCAGCCGCGGTAATACATAGGTTGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGTCTGTAGGTTGTTTATTAAGTCTGGCGTCAAAACTTGGGGCTCAACCCCAAATCGCGTTGGATACTGATAAACTAGAATTGTGTAGAGGTTAACGGAATTCCTTGTGAAGCGGTGAAATGCGTAGATATAAGGAAGAACATCAACTTGGCGAAGGCAGTTAACTGGGCACATATTGACACTGAGAGACGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGATGATTAGCTGATGGGAACCATCGGCGCAGCTAACGCATTAAATCATCCGCCTGAGTAGTATGCTCGCAAGAGTGAAACTTAAAGGAATTGACGGGGATCCGCACAAGCGGTGGAGCATGTGGTTTAATTTGAAGATACGCGTAGAACCTTACCCACTCTTGACATCTTCTGCAAAGCTATAGAGATATAGTGGAGGTTAACAGAATGACAGATGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTCGGTTAAGTCCTGCAACGAGCGCAACCCTTGTCCTTAGTTAAATATCCTAAGGAGACTGCCCGGGTAACTGGGAGGAAGGTGGGGACGACGTCAAATCATCATGCCTCTTACGAGTGGGGCAACACACGTGCTACAATGGACGGTACAAAGAGAAGCAATATGGCGACATGGAGCAAATCTCAAAAAACCGTTCTCAGTTCGGATTGTAGTCTGCAACTCGACTACATGAAGTCGGAATCGCTAGTAATCGTAGATCAGCTACGCTACGGTGAATACGTTCTCGGGTCTTGTACACACCGCCCGTCACACCATGGGAGCTGGTAATGCCCGAAGTCGGTTTAGTCAACTACGGAGAC
Fig. 2. 16S rRNA gene sequence of Mycoplasma seminis ARNOT (Accession number: EU544230)
Fig. 3. Colonies of Mycoplasma seminis ARNOT on modified Hayflick’s agar after 3 days of incubation exhibiting characteristic fried egg morphology. Bar, 1 mm. Credits: Joachim Spergser (Vetmeduni Vienna)