Mycoplasma molare
(Rosendal, 1974)
Etymology
Gr. n. mukes – fungus, Gr. neut. n. plasma – anything formed, N.L. neut. n. Mycoplasma – fungus form; L. neut. adj. molare – millstone-like (referring to heavy film production on solid media resembling patterns on the surface of millstones)
Taxonomy
Mycoplasmatales – Mycoplasmataceae – Mycoplasma – Mycoplasma molare (Hyopneumoniae cluster), closely related to Mycoplasma lagogenitalium (16S rRNA gene sequence similarity – 97.47%) (Fig. 1)
Type strain
H 542T (dog, Denmark, -1972), (Fig. 2, 16S rRNA gene sequence)
Genomes
one completed (H 542T – Denmark), one draft genome (also the type strain) (NCBI Genome deposit per 11/05/2024)
Cell morphology
spherical – coccoid
Colony morphology
fried egg morphology (Fig. 3)
Metabolism
fermentation of glucose; non-arginine-hydrolyzing, non-urea-hydrolyzing
Host
domestic dog
Habitat
throat
Disease(s)
unknown, no disease reported
Pathogenicity
factors unknown but has shown to exhibit strong sialidase activity
Epidemiology
unknown, rarely isolated from domestic dogs
Diagnosis
cultivation and species identification by MALDI-ToF MS, serology or genetically
Fig. 1. Maximum likelihood tree showing the phylogenetic position of Mycoplasma molare H 542T within the Hyopneumoniae cluster of Mycoplasmataceae based on 16S rRNA gene sequences. The sequence of Mycoplasma synoviae WVU 1853T was used as out-group (Synoviae cluster). Numbers at nodes represent bootstrap confidence values (1000 replications). Only values > 80% are shown. Bar, number of substitutions per nucleotide position. Credits: Joachim Spergser (Vetmeduni Vienna)
CTGGCTGTGTGCCTAATACATGCATGTTGAACGAAGCAGTGCTTGCACTGACTTAGTAGCGAATGGGTGAGTAACACGTACCTAACCTACCTTTAAGATTGGGATAACTATTGGAAACAGTAGCTAATACCAAATACGGTTTATTATCGCATGATGATAAATTAAAAGAAGCCTTAAAGCTTCACTTAGAAATGGGGGTGCGGAACATTAGCTAGTTGGTAGGGTAAAGGCCTACCAAGGCGATGATGTTTAGCCGGGCCGAGAGGCTGTACGGCCACACTGGGACTGAGATACGGCCCAGACTCCTACGGGAGGCAGCAGTAGGGAATTTTCCACAATGAGCGAAAGCTTGATGGAGCGACACAGCGTGCAGGATGAAGGCCTTCGGGTTGTAAACTGCTGTTGCAAGGGAAGAAAAATTTAAGGAGGAAATGCCTTAAAACTGACGGTACCTTGTTAGAAAGCGATGGCAAACTATGTGCCAGCAGCCGCGGTAATACATAGGTCGCGAGCGTTATCCGGAATTATTGGGCGTAAAGAGTTCGTAGGTTGTTTGTTAAGTCTGAAGTTAAATACTGGAGCTCAACTCCAGCCCGCTTTGGATACTGGCAAACTGGAATTACAAAGAGGTTAGCGGAATTCCTAGTGAAGCGGTGGAATGCGTAGATATTAGGAAGAACACCAATAGGCGAAGGCAGCTAACTGGTTGTATATTGACACTGAGGAACGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGTTGATCATTAGTTGGTAGAGAATTTACCGATGCAGCTAACGCATTAAATGATCCGCCTGAGTAGTATGCTCGCAAGAGTGAAACTTAAAGGAATTGACGGGGACCCGCACAAGCGGAGGAGCATGTGGTTTAATTTGAAGATACGCGTAGAACCTTACCCACTCTTGACATCCCTTGCAATGCTATAGAGATATAGCGGAGGTTAACAAGGTGACAGATGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTTGGTTAAGTCCAGCAACGAGCGCAACCCTTGTCTTTAGTAACTAGATTAAGTTGAGGACTCTAGAGAGACTGCCTGGGTAACCAGGAGGAAGGTGGGGACGACGTCAAATCATCATGCCTCTTACGAGTGGGGCAACACACGTGCTACAATGGTTGGTACAAAGAGAAGCAATATGGTGACATGGAGCAAACCTCAAAAAACCAATCTCAGTTCGGATTGAAGTCTGCAACTCGACTTCATGAAGTCGGATTCGCTAGTAATCGCAGATCAGCTACGCTGCGGTGAATACGTTCTCGGGTCTTGTACACACCGCCCGTCACACCATGGGAGTTGGTAATGCCCGAAGCCGGTTAGTTAACTTCGGAGACGACTGTCTAAGGCAGGACCGATGACTGGGGTGAAGTCGTAACAAGGT
Fig. 2. 16S rRNA gene sequence of Mycoplasma molare H 542T (Accession number: NR_114637)
Fig. 3. Colonies of Mycoplasma molare H 542T on modified Hayflick’s agar after 4 days of incubation exhibiting fried egg morphology. Bar, 1 mm. Credits: Joachim Spergser (Vetmeduni Vienna)