Mycoplasma leonicaptivi

(corr. Hill, 1992)

Etymology

Gr. n. mukes – fungus, Gr. neut. n. plasma – anything formed, N.L. neut. n. Mycoplasma – fungus form; L. n. leo -onis – a lion, L. adj. captivus – captive, N.L. gen. n. leonicaptivi – of a captive lion (corrected by Trüper & De’ Clari, 1998)

Taxonomy

Mycoplasmatales – Mycoplasmataceae – Mycoplasma – Mycoplasma leonicaptivi (Synoviae cluster), separated branch, related to Mycoplasma edwardii (16S rRNA gene sequence similarity – 95.48%) (Fig. 1)

Type strain

3L2^T (lion, UK, ≤1992), (Fig. 2, 16S rRNA gene sequence)

Genomes

one draft genome (3L2^T – UK) (NCBI Genome deposit per 11/05/2024)

Cell morphology

spherical – coccoid

Colony morphology

fried egg morphology (Fig. 3)

Metabolism

fermentation of glucose; non-arginine-hydrolyzing, non-urea-hydrolyzing

Host

lion, leopard, tiger

Habitat

throat

Disease(s)

unknow, no disease reported

Pathogenicity

factors unknown

Epidemiology

unknown, once isolated from two zoo-kept lions and a leopard, and recently from a zoo-kept tiger in Austria

Diagnosis

cultivation and species identification by MALDI-ToF MS, serology or genetically

Fig. 1. Maximum likelihood tree showing the phylogenetic position of Mycoplasma leonicaptivi 3L2^T within the Synoviae cluster of Mycoplasmataceae based on 16S rRNA gene sequences. The sequence of Mycoplasma hyopneumoniae J^T was used as out-group (Synoviae cluster). Numbers at nodes represent bootstrap confidence values (1000 replications). Only values > 80% are shown. Bar, number of substitutions per nucleotide position. Credits: Joachim Spergser (Vetmeduni Vienna)

>Mycoplasma leonicaptivi 3L2^T

CTGGCTGTGTGCCTAATACATGCATGTCGAGCGGAGTTCTTTTAGAACTTAGCGGCGAATGGGTGAGTAACACGTACTTAACGTACTCTTTAGATTGGGACAACGATGAGAAATTATCGCTAATACCGGATACTTATATTTTTCGCATGAAAGATATATAAAAGAAGCGTTCGCTTCACTAAAGAATCGGGGTGCGGAGCATTAGTTTGTTGGTAGGGTAATGGCCTACCAAGACGATGATGTTTAGCGGGGTTGAGAGACTGATCCGCCACACTGGGACTGAGATACGGCCCAGACTCCTACGGGAGGCAGCAGTAGGGAATTTTCCACAATGGGCGAAAGCCTGATGGAGCGACACAGCGTGCAGGAAGAAGGCCTTCGGGTTGTAAACTGCTGTTATAAGGGAAGAAAAAGCATTATAGGAAATGATAGTGCCTTGACGGTACCTTGTCAGAAAGCAACGGCTAACTATGTGCCAGCAGCCGCGGTAATACATAGGTTGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGTCTGTAGGTTGTGTGTTAAGTCTGGCGTTAAAACTTGGGGCTCAACCCCAAATTGCGTTGGATACTGGCATACTAGAATAGTGTAGAGGTAAGCGGAATTCCTAGTGAAGCGGTGAAATGCGTAGATATTAGGAAGAACACCAACATGGCGAAGGCAGCTTACTGGGCACTTATTGACACTGAGAGACGAAAGCGTGGGGAGCACACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGATGATTAGCTGATAGGAACTATCGGCACAGCTAACGCATTAAATCATCCGCCTGAGTAGTATGCTCGCAAGAGTGAAACTTAAAGGAATTGACGGGGATCCGCACAAGCGGTGGAGCATGTGGTTTAATTTGAAGATACGCGTAGAACCTTACCCACTCTTGACATCTTCCGCAAAGCTATAGAGATATAGTTGAGGTTAACGGAATGACAGATGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTACGCTTAAGTGCCATAACGAGCGCAACCCTTTTCTTTAGTTAATTTTTTAGAGATACTGCCCAAGTAATTGGGAGGAAGGCGGGGACGACGTCAAATCATCATGCCTCTTACGAGTGGGGCAACACACGTGCTACAATGGACGGTACAAAGAGAAGCAATATAGTGATATGGAGCAAATCTCAAAAAACCGTTCTCAGTTCGGATTGTAGTCTGCAACTCGACTACATGAAGTCGGAATCGCTAGTAATCGTAGATCAGCTACGCTACGGTGAATACGTTCTCGGGTCTTGTACACACCGCCCGTCACACCATGGGAGCTGGTAATGCCCGAAGTCGGTTTTGTTAACTACGGAGACAACTGCCTAAGGCAGGGCCGGTGACTGGGGTGAAGTCGTAACAAGGT

Fig. 2. 16S rRNA gene sequence of Mycoplasma leonicaptivi 3L2^T(Accession number: NR_025965)

Fig. 3. Colonies of Mycoplasma leonicaptivi strain 5366 (isolated from the lung of a tiger) on modified Hayflick’s agar after 4 days of incubation exhibiting characteristic fried egg morphology. Bar, 1 mm. Credits: Joachim Spergser (Vetmeduni Vienna)

Species assigned by: Hill, A.C. 1992. Mycoplasma simbae sp. nov., Mycoplasma leopharyngis sp. nov., and Mycoplasma leocaptivus sp. nov., isolated from lions. Int. J. Syst. Bacteriol. 42: 518-523.