Mycoplasma leachii

(Manso-Silván et al., 2009)

Etymology

Gr. n. mukes – fungus, Gr. neut. n. plasma – anything formed, N.L. neut. n. Mycoplasma – fungus form; N.L. masc. gen. n. leachii – of Leach (referring to R.H. Leach)

Taxonomy

Mycoplasmatales – Mycoplasmataceae – Mycoplasma – Mycoplasma leachii (Mycoides cluster), closely related to Mycoplasma mycoides and Mycoplasma capricolum (16S rRNA gene sequence similarities – 99.66 and 99.93%, respectively) (Fig. 1)

Type strain

PG50^T (cattle, Australia, 1963), (Fig. 2, 16S rRNA gene sequence)

Genomes

2 completed (PG50^T – Australia; 99/014/6 – origin undefined); one draft genome (NCBI Genome deposit per 11/05/2024)

Cell morphology

spherical– coccoid

Colony morphology

fried egg morphology (Fig. 3)

Metabolism

fermentation of glucose; assimilation of glycerol; non-arginine-hydrolyzing, non-urea-hydrolyzing

Host

cattle, occasionally isolated from goats

Habitat

udder

Disease(s)

arthritis, mastitis, abortion

Pathogenicity

pathogenicity factors unknown, but may possess factors that have been identified in other members of the Mycoides cluster

Epidemiology

isolated from cattle in Australia, occasionally isolated in Portugal, France, Germany, Nigeria, India, and Argentina; transmission primarily by milk and aerosol

Diagnosis

cultivation and species identification by MALDI-ToF MS, serology or genetically

Fig. 1. Maximum likelihood tree showing the phylogenetic position of Mycoplasma leachii PG50^T within the Mycoides cluster of Mycoplasmataceae based on 16S rRNA gene sequences. The sequence of Mycoplasma synoviae WVU 1853^T was used as out-group (Synoviae cluster). Numbers at nodes represent bootstrap confidence values (1000 replications). Only values > 80% are shown. Bar, number of substitutions per nucleotide position. Credits: Joachim Spergser (Vetmeduni Vienna)

>Mycoplasma leachii PG50^T

CTGGCGGCATGCCTAATACATGCAAGTCGAACGGGGGTGCTTGCACCTCAGTGGCGAACGGGTGAGTAACACGTATCTAACCTACCTTATAGCGGGGGATAACTTTTGGAAACGAAAGATAATACCGCATGTAGATCTTATTATCGCATGAGAAAAGATCAAAAGAACCGTTTGGTTCACTATGAGATGGGGATGCGGCGTATTAGCTAGTAGGTGAGATAATAGCCCACCTAGGCGATGATACGTAGCCGAACTGAGAGGTTGATCGGCCACATTGGGACTGAGATACGGCCCAGACTCCTACGGGAGGCAGCAGTAGGGAATTTTTCACAATGGACGAAAGTCTGATGAAGCAATGCCGCGTGAGTGATGACGGCCTTCGGGTTGTAAAGCTCTGTTGTAAGGGAAGAAAAAATAAAGTAGGAAATGACTTTATCTTGACAGTACCTTACCAGAAAGCCACGGCTAACTATGTGCCAGCAGCCGCGGTAATACATAGGTGGCAAGCGTTATCCGGATTTATTGGGCGTATAGGGTGCGTAGGCGGTTTTGCAAGTTTGAGGTTAAAGTCCGGAGCTCAACTCCGGTTCGCCTTGAAAACTGTTTTACTAGAATGCAAGAGAGGTAAGCGGAATTCCATGTGTAGCGGTGAAATGCGTAGATATATGGAAGAACACCTGTGGCGAAAGCGGCTTACTGGCTTGTTATTGACGCTGAGGCACGAAAGCGTGGGGAGCAAATAGGATTAGATACCCTAGTAGTCCACGCCGTAAACGATGAGTACTAAGTGTTGGGGTAACTCAGCGCTGCAGCTAACGCATTAAGTACTCCGCCTGAGTAGTATGCTCGCAAGAGTGAAACTCAAAGGAATTGACGGGGACCCGCACAAGTGGTGGAGCATGTGGTTTAATTCGAAGCAACACGAAGAACCTTACCAGGGCTTGACATCCAGTGCAAAGCTATAGAGATATAGTAGAGGTTAACATTGAGACAGGTGGTGCATGGTTGTCGTCAGTTCGTGCCGTGAGGTGTTGGGTTAAGTCCCGCAACGAACGCAACCCTTGTCGTTAGTTACTAACATTAAGTTGAGAACTCTAACGAGACTGCTAGTGTAAGCTAGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGTCCTGGGCTACACACGTGCTACAATGGCTGGTACAAAGAGTTGCAATCCTGTGAAGGGGAGCTAATCTCAAAAAACCAGTCTCAGTTCGGATTGAAGTCTGCAACTCGACTTCATGAAGCCGGAATCACTAGTAATCGCGAATCAGCTATGTCGCGGTGAATACGTTCTCGGGTCTTGTACACACCGCCCGTCACACCATGAGAGTTGGTAATACCAGAAGTAGGTAGATTAACCATTTGGAGAGCGCTTCCCAAGGTAGGACTAGCGATTGGGGTGAAGTCGTAACAAGGT

Fig. 2. 16S rRNA gene sequence of Mycoplasma leachii PG50^T(Accession number: NR_118797)

Fig. 3. Mycoplasma leachii PG50^T on modified Hayflick’s agar after 3 days of incubation producing large colonies with characteristic fried egg morphology. Bar, 1 mm. Credits: Joachim Spergser (Vetmeduni Vienna)

Species assigned by: Manso-Silvan, L., Vilei, E.M., Sachse, K., Djordjevic, S.P., Thiaucourt, F., Frey, J. 2009. Mycoplasma leachii sp. nov. as a new species designation for Mycoplasma sp. bovine group 7 of Leach, and reclassification of Mycoplasma mycoides subsp. mycoides LC as a serovar of Mycoplasma mycoides subsp. capri. Int. J. Syst. Evol. Microbiol. 59: 1353-1358.