Mycoplasma citelli

(Rose et al., 1978)

Etymology

Gr. n. mukes – fungus, Gr. neut. n. plasma – anything formed, N.L. neut. n. Mycoplasma – fungus form; N.L. n. Citellus – a genus of ground squirrel, N.L. gen. n. citelli – of a ground squirrel of genus Citellus

Taxonomy

Mycoplasmatales – Mycoplasmataceae – Mycoplasma – Mycoplasma citelli (Synoviae cluster), closely related to Mycoplasma columborale (16S rRNA gene sequence similarity – 97.08%) (Fig. 1)

Type strain

RG-2C^T (Richardson’s ground squirrel – Urocitellus richardsonii, Canada, 1977), (Fig. 2, 16S rRNA gene sequence)

Genomes

2 completed (2x RG-2C^T – USA) (NCBI Genome deposit per 11/05/2024)

Cell morphology

spherical

Colony morphology

fried egg morphology (Fig. 3)

Metabolism

fermentation of glucose; non-arginine-hydrolyzing, non-urea-hydrolyzing

Host

Richardson’s ground squirrel (Urocitellus richardsonii)

Habitat

respiratory tract

Disease(s)

unknown, no disease reported

Pathogenicity

factors unknown

Epidemiology

unknown, once isolated from 5 ground squirrels from Canada

Diagnosis

cultivation and species identification by MALDI-ToF MS, serology or genetically

Fig. 1. Maximum likelihood tree showing the phylogenetic position of Mycoplasma citelli RG-2C^T within the Synoviae cluster of Mycoplasmataceae based on 16S rRNA gene sequences. The sequence of Mycoplasma hyopneumoniae J^T was used as out-group (Hyopneumoniae cluster). Numbers at nodes represent bootstrap confidence values (1000 replications). Only values > 80% are shown. Bar, number of substitutions per nucleotide position. Credits: Joachim Spergser (Vetmeduni Vienna)

>Mycoplasma citelli RG-2C^T

CTGGCTGTGTGCCTAATACATGCATGTCGAGCGGAGTTTATTTATAAACTTAGCGGCGAATGGGTGAGTAACACGTACTTAACGTACCCTTTAGATTGGAATAACGCTGAGAAATTAGCGCTAATGCCGGATATTTATTACCAACGCATGTTGGTAATATCAAAGAAGCGTTTGCTTCACTAAAGGATCGGGGTGCGGAACATTAGCTAGTTGGTAAGGTAAAAGCTTACCAAGGCGATGATGTTTAGCGGGGTTGAGAGACTGAACCGCCACACTGGGACTGAGATACGGCCCAGACTCCTACGGGAGGCAGCAGTAGGGAATTTTCCACAATGAGCGAAAGCTTGATGGAGCGACACAGCGTGCAGGAGGACGGCCTTCGGGTTGTAAACTGCTGTTATAAGGGAAGAAAAAGTAGTGTAGGAAATGACATTACCTTGACGGTACCTTGTCAGAAAGCAACGGCTAACTATGTGCCAGCAGCCGCGGTAATACATAGGTTGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGTCTGTAGGTTGTTTATTAAGTCTGGCGTCAAAACTTGGGGCTCAACCCCAAATCGCGTTGGATACTGATAAACTAGAGTTATTTAGAGGTTAATGGAATTCCTTGTGAAGCGGTGGAATGCGTAGATATAAGGAAGAACACCAACATGGCGAAGGCAATTAACTGGGAATACACTGACACTGAGAGACGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGATGATTAGCTGATGGACACCATCGGCGCAGCTAACGCATTAAATCATCCGCCTGAGTAGTATGCTCGCAAGAGTGAAACTTAAAGGAATTGACGGGGATCCGCACAAGCGGTGGAGCATGTGGTTTAATTTGAAGATACGCGTAGAACCTTACCCACTCTTGACATCTTCTGCAAAGCTATAGAGATATAGTGGAGGTTAACAGAATGACAGATGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTCGGTTAAGTCCTGCAACGAGCGCAACCCTTGTCCTTAGTTAAATGTTCTAAGGAGACTGCCTAGGTAACTGGGAGGAAGGTGGGGACGACGTCAAATCATCATGCCTCTTACGAGTGGGGCAACACACGTGCTACAATGGATAGTACAAAGAGAAGCAAGATGGCAACATGGAGCAAATCTCAAAAAACTATTCTCAGTTCGGATTGAAGTCTGCAACTCGACTTCATGAAGTCGGAATCGCTAGTAATCGTAGATCAGCTACGCTACGGTGAATACGTTCTCGGGTCTTGTACACACCGCCCGTCAAACCACGGGAGCTGGTAATGCCCGAAGTCGGTTTTGTTAACTACGGAAACAACCGCCTAAGGCAGGACTGGTGACTGGGGTTAAGTCGTAACAAGGT

Fig. 2. 16S rRNA gene sequence of Mycoplasma citelli RG-2C^T(Accession number: NR_025178)

Fig. 3. Colonies of Mycoplasma citelli RG-2C^T on modified Hayflick’s agar after 4 days of incubation exhibiting fried egg morphology. Bar, 1 mm. Credits: Joachim Spergser (Vetmeduni Vienna)

Species assigned by: Rose, D.L., Tully, J.G., Langford, E.V. 1978. Mycoplasma citelli, a new species from ground squirrels. Int. J. Syst. Bacteriol. 28: 567-572.