Mycoplasma bradburyae

(Ramírez et al., 2023)

Etymology

Gr. n. mukes – fungus, Gr. neut. n. plasma – anything formed, N.L. neut. n. Mycoplasma – fungus form; NL. gen. n. bradburyae – named after Janet M. Bradbury, to honour her considerable contribution to mycoplasmology and taxonomy

Taxonomy

Mycoplasmatales Mycoplasmataceae Mycoplasma – Mycoplasma bradburyae (Pneumoniae cluster), closely related to Mycoplasma gallisepticum (16S rRNA gene sequence similarity – 99.1 %) (Fig. 1)

Type strain

T158(Yellow-legged gull – Larus michahellis, Spain, 2007), (Fig. 2, 16S rRNA gene sequence)

Genomes

2 completed (T158T – Spain; IZSVE97/66 – Italy); 5 draft genomes (NCBI Genome deposit per 11/05/2024)

Cell morphology

flask-shaped

Colony morphology

fried egg morphology (Fig. 3)

Metabolism

fermentation of glucose; non-arginine-hydrolyzing, non-urea-hydrolyzing

Host

sea birds (Yellow-legged gulls, a Kelp gull, an Italian Black-headed gull, and a Cory's shearwater) 

Habitat

upper respiratory tract

Disease(s)

so far unknown

Pathogenicity

harbour cyotadherence- and motility-related proteins (MGC2, GapA, CrmA, VlhA) 

Epidemiology

found in the tracheas of 4 seabird species

Diagnosis

cultivation and species identification by MALDI-ToF MS or genetically  

Fig. 1. Maximum likelihood tree showing the phylogenetic position of Mycoplasma bradburyae PG2within the M. pneumoniae cluster based on 16S rRNA gene sequences. The sequence of Mycoplasma synoviae WVU 1853was used as out-group (M. synoviae cluster). Numbers at nodes represent bootstrap confidence values (1000 replications). Only values > 80% are shown. Bar, number of substitutions per nucleotide position. Credits: Joachim Spergser (Vetmeduni Vienna)

>Mycoplasma bradburyae T158T

TTTTCTGAGAGTTTGATCCTGGCTCAGGATTAACGCTGGCGGCATGCCTAATACATGCAAGTCGATCGGATGTAGCAATACATTAGAGGCGAACGGGTGAGTAACACGTATCCAATCTGCCTTATAGTGGGGGATAACTAGTCGAAAGATTAGCTAATACCGCATAACAAGTTAATTATCGCATGAGAATAACTTTAAAGAAGCAACTGCTTCGCTATAAGATGAGGGTGCGGCATATCAGCTAGTTGGTGAGGGTAATGGCCCACCAAGGCGATGACGTGTAGTTATGCTGAGAGGTAGAATAACCACAATGGGACTGAGACACGGCCCATACTCCTACGGGAGGCAGCAGTAGGGAATTTTTCACAATGGACGAAAGTCTGATGGAGCAATGCCGCGTGAACGATGAAGGTCTTTTTAGATTGTAAAGTTCTTTTATTTGGGAAGAAAAGTCAGTAGAGTGGAAAGCTATTGACCTGACGGTACCATTTGAATAAGTAACGACTAACTATGTGCCAGCAGTCGCGGTAATACATAGGTTGCAAGCGTTATCCGGATTTATTGGGCGTAAAACAAGCGCAGGCGGATTAGAAAGTCTGGTGTTAAAAGCAATTGCTTAACGATTGTATGCATTGGAAACTTCTAGTCTAGAGTTTGGTAGAGAGTCCYGGAACTCCATGTGGAGCGGTGAAATGCGTAGATATATGGAAGAACACCAGAGGCGAAGGCGAGGACTTGGGCCAATACTGACGCTTAGGCTTGAAAGTGTGGGGAGCAAATAGGATTAGATACCCTAGTAGTCCACACTGTAAACGATGGATGTTAAGTGTCGGAGCGAATACTTCGGTGCTGCAGTTAACACATTAAACATCCTGCCTGAGTAGTACATTCGCAAGAATGAAACTCAAACGGAATTGACGGGGACCCGCACAAGTGGTGGAGCATGTTGCTTAATTCGACGGTACACGAAAAACCTTACCTAGACTTGACATCTTGGGCGAAGTTATAGAAATATAATGGAGGTTAACCCAATGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTATCGTTAGTTACTTTGTCTAACGAGACTGCCAACGTAAGTTGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCCTTATGTCTAGGGCTGCAAACGTGCTACAATGGCCAATACAAACAGTCGCAAACCCGTAAGGCGGAGCTAATCTGTAAAGTTGGTCTCAGTTCGGATTGAGGGCTGCAATTCGCCCTCATGAAGTCGGAATCACTAGTAATCGCGAATCAGCCATGTCGCGGTGAATACGTTCTCGGGTCTTGTACACACCGCCCGTCAAACTATGAGAGCTGGTAATATCTAAAACCGTGTTGCTAACCGCAAGGAAGCGCATGTCTAGGGTAGGGCCGGTGATTGGAGTTAAGTCGTAACAAGGTACCCCTACGAGAACGTGGGGGTGGATTACCTCCTTT

Fig. 2. 16S rRNA gene sequence of Mycoplasma bradburyae T158(Accession number: OP918915)

Fig. 3. Colonies of Mycoplasma bradburyae T158T on modified SP4 agar after 5 days of incubation exhibiting characteristic fried egg morphology. Bar, 1 mm. Credits: Joachim Spergser (Vetmeduni Vienna)

Species assigned by: Ramírez, A.S., Poveda, J.B., Dijkman, R., Poveda, C., Suárez-Pérez, A., Rosales, R.S., Feberwee, A., Szostak, M.P., Ressel, L., Viver, T., Calabuig, P., Catania, S., Gobbo, F., Timofte, D., Spergser, J. 2023. Mycoplasma bradburyae sp. nov. isolated from the trachea of sea birds. Syst. Appl. Microbiol. 46: 126472.

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