Mycoplasma bovoculi

(Langford & Leach, 1973)

Etymology

Gr. n. mukes – fungus, Gr. neut. n. plasma – anything formed, N.L. neut. n. Mycoplasma – fungus form; L. n. bos – of the ox, bull, cow, L. n. oculus – the eye, N.L. gen. n. bovoculi – of the bovine eye

Taxonomy

Mycoplasmatales – Mycoplasmataceae – Mycoplasma – Mycoplasma bovoculi (Hyopneumoniae cluster), related to Mycoplasma conjunctivae (16S rRNA gene sequence similarity – 95.23%) (Fig. 1)

Type strain

M165/69^T (cattle, Canada, 1969), (Fig. 2, 16S rRNA gene sequence)

Genomes

one completed (M165/69^T – Canada) (NCBI Genome deposit per 11/05/2024)

Cell morphology

spherical – coccoid

Colony morphology

fried egg morphology (Fig. 3)

Metabolism

fermentation of glucose; non-arginine-hydrolyzing, non-urea-hydrolyzing

Host

cattle

Habitat

eye

Disease(s)

Infectious Keratoconjunctivitis

Pathogenicity

factors unknown

Epidemiology

worldwide occurrence; transmission through close contact and flying insects

Diagnosis

cultivation and species identification by MALDI-ToF MS, serology or genetically; PCR; detection of antibodies using immunoassays for herd testing

Fig. 1. Maximum likelihood tree showing the phylogenetic position of Mycoplasma bovoculi M165/69^T within the Hyopneumoniae cluster of Mycoplasmataceae based on 16S rRNA gene sequences. The sequence of Mycoplasma synoviae WVU 1853^T was used as out-group (Synoviae cluster). Numbers at nodes represent bootstrap confidence values (1000 replications). Only values > 80% are shown. Bar, number of substitutions per nucleotide position. Credits: Joachim Spergser (Vetmeduni Vienna)

>Mycoplasma bovoculi M165/69^T

CTCGCTGTGTGCCTAATACATGCATGTTGAACGGAATATTTTAGCTTGCTAAAATATTTAGTAGCGAATGGGTGAGTAACACGTACCTAATCTACCTTTAGGTTCGGGATAACTTTTGGAAACAAGAGCTAATACCTGATATAGTTTATTTTCGCATGAAGATAAATAAAAAGGAGCTTCACGGCTTCGCCTAGAAATGAGGGTGCGGAACATTAGTTAGTTGGTGAGGTAATGGCTCACCAAGACGATGATGTTTAGCGGGGCCAAGAGGTTGTACCGCCACACTGGGACTGAGATACGGCCCAGACTCCTACGGGAGGCAGCAGTAAGGAATTTTCCACAATGAGCGAAAGCTTGATGGAGCGACACAGCGTGCACGATGAAGTCTTTAGGGATGTAAAGTGCTGTTATAAGGAAAGAAAAAATTAAATAGGAAATGATTTAATCTTGACGGTACCTTATTAGAAAGCGACGGCAAACTATGTGCCAGCAGCCGCGGTAATACATAGGTCGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGTCCGTAGGTGTTTTGTTAAGTTTAAGGTTAAATACTGAAGCTCAACTTCAGCCCGCTTTAGATACTGGCAAAATAGAATTATGAAGAGGTTAGCGGAATTCCTAGTGGAGCGGTGGAATGCGTAGATATTAGGAAGAACACCAATAGGCGAAGGCAGCTAACTGGTCATAAATTGACACTAAGGGACGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCTGTAAACGATGATCATTAGTTGGTGGTAATAATCACTAGCACAGCTAACGCGATAAATGATCCGCCTGAGTAGTATGCTCGCAAGAGTGAAACTTAAAGGAATTGACGGGAACCCGCACAAGCGGTGGAGCATGTGGTTTAATTTGAAGATACGCGGAGAACCTTACCCACTCTTGACATCCTTGCAAAGCTATAGAGATATAGTAGAGGTTAACAAGATGACAGATGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTAGGTTAAGTCCTGCAACGAGCGCAACCCCTTTCTTTAGTTACTAACATTAAGTTGAGGACTCTAGAGACACTGCCAGCGTAAGCTGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCTCTTACGAGTGGGGCAACACACGTGCTACAATGGCTACTACAAAGAGAAGCAAAACGGTGACGTCCAGCAAATCTCAAAAAAGTAGTCCCAGTTCGGATTGAAGTCTGCAACTCGACTTCATGAAGTCGGAATCGCTAGTAATCGCAGGTCAGCTATACTGCGGTGAATACGTTCTCGGGTTTTGTACACACCGCCCGTCACACCATGGGAGTTGGTAATACCCAAAGTCGGTTAGTTAACTTCGGAGACTGCTGCCTAAGGTAGGACTGATGACTGGGGTGAAGTCGTAACAAGGT

Fig. 2. 16S rRNA gene sequence of Mycoplasma bovoculi M165/69^T(Accession number: NR_121731)

Fig. 3. Colonies of Mycoplasma bovoculi M165/69^T on modified Hayflick’s agar after 5 days of incubation exhibiting characteristic fried egg morphology and variable colony sizes. Bar, 1 mm. Credits: Joachim Spergser (Vetmeduni Vienna)

Species assigned by: Langford, E.V., Leach, RH. 1973. Characterization of a mycoplasma isolated from infectious bovine keratoconjunctivitis: M. bovoculi sp. nov. Can. J. Microbiol. 19: 1435-1444.