Mycoplasma anserisalpingitidis

(Volokhov et al., 2020)

Etymology

Gr. n. mukes – fungus, Gr. neut. n. plasma – anything formed, N.L. neut. n. Mycoplasma – fungus form; N.L. masc. n. anser – a goose, N.L. fem. n. salpingitis – inflammation of the oviduct, N.L. gen. n. anserisalpingitidis - of an inflamed oviduct of a goose

Taxonomy

MycoplasmatalesMycoplasmataceaeMycoplasmaMycoplasma anserisalpingitidis (Synoviae cluster), closely related to Mycoplasma anatis (16S rRNA gene sequence similarity – 99.17%) (Fig. 1)

Type strain

1220T (goose, Hungary, ≤1984), (Fig. 2, 16S rRNA gene sequence)

Genomes

5 completed (1220T – Hungary, MYCAV 93 – Hungary, MYCAV 177 – Hungary, MYCAV 271 - Hungary, MA 271 – Hungary) (NCBI Genome deposit per 11/05/2024)

Cell morphology

spherical – coccoid

Colony morphology

fried egg morphology (Fig. 3)

Metabolism

fermentation of glucose and fructose; non-arginine-hydrolyzing, non-urea-hydrolyzing

Host

goose but have also been isolated from ducks

Habitat

cloaca, reproductive organs

Disease(s)

associated with inflammation of the cloaca and phallus, salpingitis, decreased egg production and embryo mortality

Pathogenicity

factors unknow

Epidemiology

probably worldwide occurrence in geese; transmission probably by the genital route, vertical transmission to eggs

Diagnosis

cultivation and species identification by MALDI-ToF MS, serology or genetically, PCR

Fig. 1. Maximum likelihood tree showing the phylogenetic position of Mycoplasma anserisalpingitidis 1220T within the Synoviae cluster of Mycoplasmataceae based on 16S rRNA gene sequences. The sequence of Mycoplasma hyopneumoniae JT was used as out-group (Hyopneumoniae cluster). Numbers at nodes represent bootstrap confidence values (1000 replications). Only values > 80% are shown. Bar, number of substitutions per nucleotide position. Credits: Joachim Spergser (Vetmeduni Vienna)

>Mycoplasma anserisalpingitidis 1220T

CTGGCTGTGTGCCTAATACATGCATGTCGAGCGGAGTTCTTCGGAACTTAGCGGCGAATGGGTGAGTAACACGTACTTAACATGCCCTCTAGATTGGAATAACGATTAGAAATGATCGCTAATGCCGGATACTTATATCTTTCGCATGAAGGATATATAAAAGGAGCGTTTGCTTCGCTAGAGGATTGGGGTGCGTAACATTAGCTAGTTGGTAGGGTAATGGCCTACCAAGGCTATGATGTTTAGCGGGGTTGAGAGACTGATCCGCCACACTGGGACTGAGATACGGCCCAGACTCCTACGGGAGGCAGCAGTAGGGAATTTTCCACAATGGACGAAAGTCTGATGGAGCGACACAGCGTGCAGGATGACGGCCTTCGGGTTGTAAACTGCTGTTATAAGGGAAGAAAAAATAAAGTAGGAAATGACTTTATCTTGACGGTACCTTGTCAGAAAGCAACGGCTAACTATGTGCCAGCAGCCGCGGTAATACATAGGTTGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGTCTGTAGGTTGTTTGTTAAGTCTGACGTGAAAACTTGGGGCTCAACCCCAAATTGCGTTGGATACTGGCAAACTAGAGTTATGAAGAGGTTAGCGGAATTCCTTGTGAAGCGGTGAAATGCGTAGATATAAGGAAGAACACCAACTTGGCGAAGGCAGCTAACTGGGCATACACTGACACTGAGAGACGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCTGTAAACGATGATGATTAGCTGATGGGGAACTCATCGGCACAGCTAACGCATTAAATCATCCGCCTGAGTAGTATGCTCGCAAGAGTGAAACTTAAAGGAATTGACGGGGATCCGCACAAGCGGTGGAGCATGTGGTTTAATTTGAAGATACGCGTAGAACCTTACCCACTCTTGACATCTTCCGCAAAGCTATAGAGATATAGTGGAGGCTAACGGAATGACAGATGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTCGGTTAAGTCCTGCAACGAGCGCAACCCTTGTCCTTAGTTAAATGTTCTAAGGAGACTGCCCGAGTAATTGGGAGGAAGGTGGGGACGACGTCAAATCATCATGCCTCTTACGAGKGGGGCAACACACGTGCTACAATGGACGGTACAAAGAGACGCAATACGGCGACGTGGAGCAAATCTCAAAAAACCGTTCTCAGTTCGGATTGTAGTCTGCAACTCGACTACATGAAGTCGGAATCGCTAGTAATCGTAGATCAGCTACGCTACGGTGAATACGTTCTCGGGTCTTGTACACACCGCCCGTCACACCATGGGAGCTGGTAATGCCCGAAGTCGGTTTTGTTAACTACGGAGACAACTGCCTAAGGCAGGACTGGTGACTGGGGTGAAGTCGTAACAAGGT

Fig. 2. 16S rRNA gene sequence of Mycoplasma anserisalpingitidis 1220(Accession number: EU597750)

Fig. 3. Colonies of Mycoplasma anserisalpingitidis strain 455 (isolated from the phallus of a gander) on modified Hayflick’s agar after 4 days of incubation exhibiting fried egg morphology. Bar, 1 mm. Credits: Joachim Spergser (Vetmeduni Vienna)

Species assigned by: Volokhov, D.V., Grozner, D., Gyuranecz, M., Ferguson-Noel, N., Gao, Y., Bradbury, J.M., Whittaker, P., Chizhikov, V.E., Szathmary, S., Stipkovits, L. 2020. Mycoplasma anserisalpingitidis sp. nov., isolated from European domestic geese (Anser anser domesticus) with reproductive pathology. Int. J. Syst. Evol. Microbiol. 70: 2369-2381. 

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