Mycoplasma procyoni

(Volokhov et al., 2019)

Etymology

Gr. n. mukes – fungus, Gr. neut. n. plasma – anything formed, N.L. neut. n. Mycoplasma – fungus form; L. n. procyon – genus name of the racoon, L. gen. n. procyoni – of a racoon

Taxonomy

Mycoplasmatales – Mycoplasmataceae – Mycoplasma – Mycoplasma procyoni (Hyopneumoniae cluster), related to Mycoplasma lagogentialium (16S rRNA gene sequence similarity – 96.98%) (Fig. 1)

Type strain

LR5794^T (racoon – Procyon lotor, Canada, 2005?), (Fig. 2, 16S rRNA gene sequence)

Genomes

one draft genome (LR5784^T – Canada) (NCBI Genome deposit per 11/05/2024)

Cell morphology

spherical – coccoid

Colony morphology

fried egg morphology (Fig. 3)

Metabolism

fermentation of glucose; non-arginine hydrolyzing, non-urea-hydrolyzing

Host

racoon (Procyon lotor)

Habitat

oral cavity

Disease(s)

unknown, no disease reported

Pathogenicity

factors unknown

Epidemiology

unknown, once isolated from the oral cavity of a racoon from Canada

Diagnosis

cultivation and species identification by MALDI-ToF MS or genetically

Fig. 1. Maximum likelihood tree showing the phylogenetic positions of Mycoplasma procyoni LR5794^T within the Hyopneumoniae cluster of Mycoplasmataceae based on 16S rRNA gene sequences. The sequence of Mycoplasma synoviae WVU 1853^T was used as out-group (Synoviae cluster). Numbers at nodes represent bootstrap confidence values (1000 replications). Only values > 80% are shown. Bar, number of substitutions per nucleotide position. Credits: Joachim Spergser (Vetmeduni Vienna)

>Mycoplasma procyoni LR5794^T

TTAACGCTGGCTGTGTGCCTAATACATGCATGTTGAACGAAGCAGTGCTTGCACTGACTTAGTAGCGAATGGGTGAGTAACACGTACCTAACCTACCTTTTAGATTGGGATAACTACTGGAAACAGTAGCTAATACCGAATACGAATTTCTGTCGCATGATGGAAGTTTAAAAGGAGCCTCAAAGCTTCACTTAAAGATGGGGGTGCGGAACATTAGCTAGTTGGTAGGGTAATGGCCTACCAAGGCGATGATGTTTAGCCGGGCCGAGAGGCTGTACGGCCACACTGGGACTGAGATACGGCCCAGACTCCTACGGGAGGCAGCAGTAGGGAATTTTCCACAATGAGCGAAAGCTTGATGGAGCGACACAGCGTGCAGGATGAAGGCCTTCGGGTTGTAAACTGCTGTTGCAAGGGAAGAAAAAATAGGGTAGGAAATGAYCCTTAATTGACGGTACCTTGTTAGAAAGCGATGGCAAACTATGTGCCAGCAGCCGCGGTAATACATAGGTCGCGAGCGTTATCCGGAATTATTGGGCGTAAAGAGTTCGTAGGTTGTTTGTTAAGTCTGGAGTTAAATACCGAAGCTCAACTTCGGCCCGCTTTAGATACTGGCAAGCTAGAATTACAAAGAGGTTAGCGGAATTCCTAGTGAAGCGGTGGAATGCGTAGATATTAGGAAGAACACCAATAGGCGAAGGCAGCTAACTGGTTGTATATTGACACTGAGGAACGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGTTGATCATTAGTTGGTAGGAAGACTTACCGATGCAGCTAACGCAGTAAATGATCCGCCTGAGTAGTATGCTCGCAAGAGTGAAACTTAAAGGAATTGACGGGGACCCGCACAAGCGGAGGAGCATGTGGTTTAATTTGAAGATACGCGTAGAACCTTACCCACTCTTGACATCCCTTGCAATGCTATAGAGATATAGCGGAGGTTAACAAGGTGACAGATGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTAGGTTAAGTCCTGCAACGAGCGCAACCCTTGTCTTTAGTTACTAACATTTAGTTGAGGACTCTAGAGAGACTGCCTGGGTAACCAGGAGGAAGGTGGGGACGACGTCAAATCATCATGCCTCTTACGAGTGGGGCAACACACGTGCTACAATGGTTGGTACAAAGAGAAGCAATATGGTGACATGGAGCAAACCTCAAAAAACCAATCTCAGTTCGGATTGAAGTCTGCAACTCGACTTCATGAAGTCGGATTCGCTAGTAATCGCAGATCAGCTACGCTGCGGTGAATACGTTCTCGGGTCTTGTACACACCGCCCGTCACACCATGGGAGTTGGTAATGCCCGAAGCCGGTTAGTTAACTTCGGAGACGACTGTCTAAGGCAGGACCGATGACTGGGGTGAAGTCGTAACAAGGT

Fig. 2. 16S rRNA gene sequence of Mycoplasma procyoni LR5794^T(Accession number: FJ226566)

Fig. 3. Colonies of Mycoplasma procyoni LR5794^T on modified Hayflick’s agar after 4 days of incubation exhibiting fried egg morphology. Bar, 1 mm. Credits: Joachim Spergser (Vetmeduni Vienna)

Species assigned by: Volokhov, D.V., Gao, Y., Davidson, M.K., Chizhikov, V.E. 2020. Acholeplasma equirhinis sp. nov. isolated from respiratory tract of horse (Equus caballus) and Mycoplasma procyoni sp. nov. isolated from oral cavity of raccoon (Procyon lotor). Arch. Microbiol. 202: 411-420.