Mycoplasma neophronis

(Suárez-Pérez et al., 2012)

Etymology

Gr. n. mukes – fungus, Gr. neut. n. plasma – anything formed, N.L. neut. n. Mycoplasma – fungus form; N.L. n. Neophron – a genus name of vultures, N.L. gen. n. neophronis – of Neophron

Taxonomy

Mycoplasmatales – Mycoplasmataceae – Mycoplasma – Mycoplasma neophronis (Hominis cluster), closely related to Mycoplasma spumans (16S rRNA gene sequence similarity – 98.70%) (Fig. 1)

Type strain

G.A.^T (Canarian Egyptian vulture – Neophron percnopterus majorensis, Spain, 2003), (Fig. 2, 16S rRNA gene sequence)

Genomes

one draft genome (G.A.^T – Spain) (NCBI Genome deposit per 11/05/2024)

Cell morphology

spherical – coccoid

Colony morphology

fried egg morphology (Fig. 3)

Metabolism

hydrolysis of arginine; non-fermentative, non-urea-hydrolyzing

Host

Canarian Egyptian vulture (Neophron percnopterus majorensis)

Habitat

upper respiratory tract

Disease(s)

unknown, no disease reported

Pathogenicity

factors unknown

Epidemiology

unknown, has once been isolated from 6 vultures in Gran Canaria, Spain

Diagnosis

cultivation and species identification by MALDI-ToF MS, serology or genetically

Fig. 1. Maximum likelihood tree showing the phylogenetic position of Mycoplasma neophronis G.A.^T within the Hominis cluster of Mycoplasmataceae based on 16S rRNA gene sequences. The sequence of Mycoplasma synoviae WVU 1853^T was used as out-group (Synoviae cluster). Numbers at nodes represent bootstrap confidence values (1000 replications). Only values > 80% are shown. Bar, number of substitutions per nucleotide position. Credits: Joachim Spergser (Vetmeduni Vienna)

>Mycoplasma neophronis G.A.^T

CTGGCTGTGTGCCTAATACATGCATGTCCGAGCGAGGTTCTTCGGAACCTAGCGGCGAATGGGTGAGTAACACGTGCTTAATCTACCCTTCAGATTGGAATACCCAATGGAAACATTGGCTAATGCCGGATACGCATGGAATCGCATGATTCCGTTGTGAAAGGGGCCTTTAAAGCCCCACTGAAGGATGAGGGTGCGGAACATTAGTTAGTTGGTAGGGTAATGGCCTACCAAGACTATGATGTTTAGCCGGGTCGAGAGACTGAACGGCCACATTGGGACTGAGATACGGCCCAAACTCCTACGGGAGGCAGCAGTAGGGAATATTCCACAATGAGCGAAAGCTTGATGGAGCGACACAGCGTGCACGATGAAGGTCTTCGGATTGTAAAGTGCTGTTATAAGGGAAGAACACTACATTGAGGAAATGCTTTGTAGCTGACGGTACCTTGTCAGAAAGCGATGGCTAACTATGTGCCAGCAGCCGCGGTAATACATAGGTCGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGTTCGTAGGCTGTTTATTAAGTCTGGAGTCAAATCCCGGGGCTCAACCCCGGCTCGCTTTGGATACTGATAAACTAGAGTTGGATAGAGGTAAGCGGAATTCCATGTGAAGCGGTGAAATGCGTAGATATATGGAAGAACACCAAAGGCGAAGGCAGCTTACTGGGTCTATACTGACGCTGAGGGACGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGATCATTAGTCGGTGGAGAGTTCACTGACGCAGCTAACGCATTAAATGATCCGCCTGAGTAGTATGCTCGCAAGAGTGAAACTTAAAGGAATTGACGGGGACCCGCACAAGCGGTGGAGCATGTGGTTTAATTTGAAGATACACGGAGAACCTTACCCACTCTTGACATCTTCCGCAAAGCTATAGAGATATAGTGGAGGCTAACGGAATGACAGATGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTTGGTCAAGTCCTGCAACGAGCGCAACCCCTATCTTTAGTTACTAACGAGTCATGTCGAGGACTCTAGAGATACTGCCTGGGTAACCGGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCTCTTACGAGTGGGGCCACACACGTGCTACAATGGTCGGTACAAAGAGAAGCAATATGGCGACATGGAGCAAATCTCAAAAAGCCGATCTCAGTTCGGATTGGAGTCTGCAATTCGACTCCATGAAGTCGGAATCGCTAGTAATCGCAGATCAGCTATGCTGCGGTGAATACGTTCTCGGGTCTTGTACACACCGCCCGTCACACCATGGGAGCTGGTAATACCCAAAGTCGGTTTGCTAACCTCGGAGGCGACCGCCTAAGGTAGGACTGGTGACTGGGGTGAAGTCGTAACAAGGT

Fig. 2. 16S rRNA gene sequence of Mycoplasma neophronis G.A.^T(Accession number: NR_108494)

Fig. 3. Colonies of Mycoplasma neophronis G.A.^T on modified Hayflick’s agar after 4 days of incubation exhibiting fried egg morphology. Note, colour change of solid medium from ochre to reddish based on release of ammonia resulting from hydrolysis of arginine creating an alkaline pH. Bar, 1 mm. Credits: Joachim Spergser (Vetmeduni Vienna)

Species assigned by: Suárez-Pérez, A., Ramírez, A.S., Rosales, R.S., Calabuig, P., Poveda, C., Rossello-Mora, R., Nicholas, R.A., Poveda, J.B. 2012. Mycoplasma neophronis sp. nov., isolated from the upper respiratory tract of Canarian Egyptian vultures (Neophron percnopterus majorensis). Int. J. Syst. Evol. Microbiol. 62: 1321-1325.