Mycoplasma nasistruthionis

(Spergser et al., 2020)

Etymology

Gr. n. mukes – fungus, Gr. neut. n. plasma – anything formed, N.L. neut. n. Mycoplasma – fungus form; L. masc. n. nasus – the nose, L. n. struthio – the ostrich, N.L. gen. n. nasistruthionis – of the nose of an ostrich

Taxonomy

Mycoplasmatales – Mycoplasmataceae – Mycoplasma – Mycoplasma nasistruthionis (Synoviae cluster), related to Mycoplasma verecundum (16S rRNA gene sequence similarity – 94.92%) (Fig. 1)

Type strain

2F1A^T (ostrich – Struthio camelus, Namibia, 2005), (Fig. 2, 16S rRNA gene sequence)

Genomes

two completed genomes (2F1A^T – Namibia; Ms03 – South Africa) (NCBI Genome deposit per 11/05/2024)

Cell morphology

spherical – coccoid

Colony morphology

fried egg morphology (Fig. 3)

Metabolism

oxidation of organic acids (pyruvate, lactate); non-fermentative, non-arginine-hydrolyzing, non-urea-hydrolyzing

Host

ostrich (Struthio camelus)

Habitat

respiratory tract

Disease(s)

associated with respiratory disease and disorders

Pathogenicity

unknown

Epidemiology

unknown, once isolated from ostriches in South Africa and Namibia

Diagnosis

cultivation and species identification by MALDI-ToF MS, serology or genetically

Fig. 1. Maximum likelihood tree showing the phylogenetic position of Mycoplasma nasistruthionis 2F1A^T within the Synoviae cluster of Mycoplasmataceae based on 16S rRNA gene sequences. The sequence of Mycoplasma hyopneumoniae J^T was used as out-group (Hyopneumoniae cluster). Numbers at nodes represent bootstrap confidence values (1000 replications). Only values > 80% are shown. Bar, number of substitutions per nucleotide position. Credits: Joachim Spergser (Vetmeduni Vienna)

>Mycoplasma nasistruthionis 2F1A^T

CTGGCTGTGTGCCTAATACATGCATGTCGAGCGGAGTTCTTCGGAACTTAGCGGCGAATGGGTGAGTAACACGTACTTAACGTGCCTTTTAGACTGGAATAACACTGAGAAATTAGTGCTAATGCCGGATACTTATACAAATCGCATGATTTGTATATGAAAGAAGCCTCAAAGCTTCACTAAAAGATCGGGGTGCGTAACATTAGCTAGTTGGTGAGGTAACGGCCCACCAAGGCTATGATGTTTAGCGGGGTTGAGAGACCGAACCGCCACACTGGGACTGAGATACGGCCCAGACTCCTACGGGAGGCAGCAGTAGGGAATTTTCCACAATGGGCGAAAGCCTGATGGAGCGACACAGCGTGCAGGAAGACGGCCTTCGGGTTGTAAACTGCTGTTATTTAGGATGAAAAAACCTTGCAGGAAATGGCAAGGCCTTGACAGTACTAAATCAGAAAGCAACGGCTAACTATGTGCCAGCAGCCGCGGTAATACATAGGTTGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGTCTGTAGGTTGTGTGTTAAGTCTGGCGTCAAAACTTGGGGCTCAACCCCAAATCGCGTTGGATACTGGCACGCTAGAATTGTATAGAGGTTAACGGAATTCCTTGTGAAGCGGTGAAATGCGTAGATATAAGGAAGAACACCAACATGGCGAAGGCAGTTAACTGGGTACATATTGACACTGAGAGACGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCTGTAAACGATGATGATTAGCTGATAGGAACTATCGGCGCAGCTAACGCATTAAATCATCCGCCTGAGTAGTATGCTCGCAAGAGTGAAACTTAAAGGAATTGACGGGGATCCGCACAAGCGGTGGAGCATGTGGTTTAATTTGAAGATACGCGTAGAACCTTACCCACTCTTGACATCTTCTGCAAAGCTATAGAGATATAGTGGAGGTCAACAGAATGACAGATGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTCGGTTAAGTCCTGCAACGAGCGCAACCCTTATTCTTAGTTAAATCTTCTAGGGAGACTGCCCGGGTAACCGGGAGGAAGGTGGGGACGACGTCAAATCATCATGCCTCTTACGAGTGGGGCAACACACGTGCTACAATGGATGGTACAAAGAGAAGCAATACGGCGACGTGGAGCAAATCTCAAAAAACCATTCTCAGTTCGGATTGTAGTCTGCAACTCGACTACATGAAGTCGGAATCGCTAGTAATCGTAGATCAGCTACGCTACGGTGAATACGTTCTCGGGTCTTGTACACACCGCCCGTCACACCATGGGAGCTGGTAATGCCCGAAGTCGGTTTTGTTAACTACGGAAACAACTGCCTAAGGCAGGACTGGTGACTGGGGTGAAGTCGTAACAAGGT

Fig. 2. 16S rRNA gene sequence of Mycoplasma nasistruthionis 2F1A^T(Accession number: FM165076)

Fig. 3. Colonies of Mycoplasma nasistruthionis 2F1A^T on modified Hayflick’s agar after 4 days of incubation exhibiting fried egg morphology. Bar, 1 mm. Credits: Joachim Spergser (Vetmeduni Vienna)

Species assigned by: Spergser, J., Botes, A., Nel, T., Ruppitsch, W., Lepuschitz, S., Langer, S., Ries, S., Dinhopl, N., Szostak, M., Loncaric, I., Busse, H.-J. Mycoplasma nasistruthionis sp. nov. and Mycoplasma struthionis sp. nov. isolated from ostriches with respiratory disease. Syst. Appl. Microbiol. 43: 126047.